A minimum order of just one hundred dollars We offer many kinds you can pick from. Dicaffeoylquinic acid, chlorogenic acid, … IHFE did not exhibit relevant cytotoxicity for both cancerous and normal cells according to dose-response curves (Fig. [ Links ], BROWN, B. J.; MITCHELL, R. J.; GRAHAM, S. Competition for pollination between an invasive species (purple loosestrife) and a native congener. In three independent experiments, the levels of IL-8 in IHFE-stimulated and non-stimulated THP-1 cells were similar, which suggests the absence of a sensitizing effect. This greater richness of pollinators in weed flowers can be related to reward offer at that site (Table 1), considering that in open cultivated areas weeds may be the only available resource, at some particular times. A standardized reference value of CC50 is not available to estimate the cytotoxic potential of plant extracts. Undamaged, fully developed flowers were dried in a VirTis AdVantage Plus tray lyophilizer (Gardiner, USA). Confluent monolayers of cells in 96-well cell culture microplate were treated with IHFE at concentrations in the range of 50 to 500 μg/ml for 3 days at 37 °C in a 5% CO2 humidified atmosphere. This flowering behavior may be associated to the avoidance of competition by pollinators, as pointed out by Gentry (1974a, 1974b) in relation to bignon species that share pollinators. For each visitor, abundance was registered considering the number of recorded visits. Google Scholar, Otsuki T, Matsufuji H, Takeda M, Toyoda M, Goda Y (2002) Acylated anthocyanins from red radish (Raphanus sativus L.). In groups C and D, the dorsal area of each mouse was divided into two and Parsol® (100 μl, 30 mg/ml) and IHFE (100 μl, 30 mg/ml) were applied on the left and right zones, respectively. In Botucatu, 30 individual flower buds of I. grandifolia and I. nil, both from ten individuals, received the same treatment and, for the three species, other 30 flowers from five individuals were marked and maintained uncovered to estimate natural fruit-set (control). Qualitative data analysis of pollinators (presence/absence) on each Ipomoea was performed using the Jacquard coefficient for similarity evaluation (Sneath & Sokal, 1973). Efforts to improve breeding of sweetpotato for traits important to small farmers can be accelerated through the use of genomics-assisted (genomics-guided) breeding. Coefficients of determination (R2 = 0.9896–0.9990) demonstrated good method linearity in the range of concentrations evaluated (2–10 μg/ml). The version 3 pseudomolecules, annotation, and RNA-seq gene expression data are available to download below. 2007). Six cell lines were selected (Table S1, Supplementary material), which represented cells from hepatocellular carcinoma (HepG-2), leukemia (THP-1), normal kidney (HEK-293), normal lung (MCR-5), and xeroderma pigmentosum (XP4PA and XP12RO-SV). 2010). Sweet Potato Vines are a wonderful addition to gardens and containers, with their rich, heart-shaped leaves and trailing growth. A standardized reference value of effective dose 50 in the THP-1/IL-8 assay is not available to estimate the sensitizing effect of plant extracts. Turrialba, v. 41, p. 344-349, 1991. 2012). Anthesis started around 05:00h and lasted mostly until afternoon (Table 2), when the corollas wilted and fell off. JCB and MRG contributed to histological analysis. In general, higher similarity was observed between different plant species in the same locality than between plant populations of the same species in different localities. In: BURLEY, J.; STYLES, B. T. Essential Ipomoea floral traits (Table 2, Figure 2) reveal that several of them are characteristics of melittophily, as described by Faegri & Pijl (1979). New York: Academic Press, 1965. p. 147-172. The mixture was filtered, and the residue was extracted twice more. The production of interleukin (IL)-8 by stimulated THP-1 cells is used as a biomarker of sensitizing effect (Takahashi et al. Extension of lesions evaluated in the skin of the BALB/c mice treated with ethanol (70%, v/v), Parsol®, or Ipomea horsfalliae flower extract (3 mg/ml), exposed to UV-B. 1974a. (2019). The US National Cancer Program suggests a cut-off for the effective dose 50 of 30 μg/ml in cell-based assays used to select plant extracts with potential anticancer effect (WHO 2019). Polinização em Ipomoea cairica (L.) Sweeth (Convolvulaceae). 2005). Nectar was the main reward for I. grandifolia and I. nil visitors since pollen collection was rarely observed in these flowers. [ Links ], ALOISI, R. R.; DEMATTÉ, J. L. I. Levantamento dos solos da Faculdade de Medicina Veterinária e Agronomia de Jaboticabal. In allogamous or facultative autogamous weed species, pollination is performed by the wind or by non-specialized pollinators (Baker, 1965). Mo. (Eds.). https://doi.org/10.1016/j.fitote.2016.09.003, Rivelli DP, Filho CA, Almeida RL, Ropke CD, Sawada TC, Barros SB (2010) Chlorogenic acid UVA-UVB photostability. 2003). This arrangement enhances spontaneous self-pollination because some stamens touch the stigma. Res. have applications in medicine, due to their hypotensive, antimicrobial, anticancer (Bieber et al. Uses Containers, Flower Beds, Hanging Baskets Care. In this work, we studied the potential photoprotective effect of Ipomoea horsfalliae Hook., Convolvulaceae, flower extract. Folia Biol (Praha) 49:197–202, CAS Some limitations of this study need to be considered. He defined this phenological pattern as "cornucopia", which can be applied to the studied Ipomoea, specially I. grandifolia and I. nil. Spring Matrix Yellow Blotch has bright yellow flowers with dark centers. PubMed In contrast, THP-1 cells stimulated with IHFE at a concentration of 80 μg/ml did not increase the IL-8 production (204 ± 54 pg/ml) which suggests the lack of potential sensitizing effect at the concentration tested. [ Links ], BAKER, H. G. Characteristics and modes of origin of weeds. Mass range in all experiments was set at m/z 80–1000. Ministry of Environment, Housing and Territorial Development and Research Institute of Biological Resources Alexander von Humboldt, Bogotá, Bieber LW, Da Silva Filho ÁA, Corréa RM, Chiappeta ADA, Do Nascimento SC, De Souza IA, De Méllo JF, Veith HJ (1986) Anticancer and antimicrobial glycosides from Ipomoea bahiensis. 2018), and vasodilator (Kwon et al. https://doi.org/10.1111/j.1751-1097.2010.00815.x, CAS Evolution, v. 31, p. 32-46, 1977. A higher number of congested blood vessels, extravasation of erythrocytes, and polymorphonuclear cells were observed in the dermis. Matrix True Blue Pansy has clear, purplish blue flowers. In these studies, each species showed a well-defined set of visitors but there was some overlapping. In addition, IHFE did not exhibit a relevant cytotoxic effect on cells derived from both normal kidney and lung, which are target cell models used in predictive toxicology testing. This hardy flower will tolerate cold and inclement weather and provide year round color for your garden. Flower extracts were analyzed on an UHPLC Dionex™ UltiMate™ 3000 (Thermo Fisher Scientific TFS, Bremen, Germany), coupled to an Orbitrap™ mass detector (Exactive Plus, TFS, Bremen, Germany), using a heated-electrospray interface (HESI-II), operated in positive ion mode (350 °C). Toxicol Sci 124:359–369. There are two sweetpotato germplasm collections (Plant Genetic Resources Conservation Unit and US Vegetable Laboratory) maintained by the USDA, ARS for sweetpotato crop improvement. Their phenolic compounds can scavenge reactive oxygen species, reduce skin alterations caused by UV exposure, and prevent aging (Estrella-Parra et al. PubMed Central Google Scholar, Afaq F, Khan N, Syed DN, Mukhtar H (2010) Oral feeding of pomegranate fruit extract inhibits early biomarkers of UVB radiation-induced carcinogenesis in SKH-1 hairless mouse epidermis. https://doi.org/10.1111/j.1751-1097.2010.00776.x, Takahashi T, Kimura Y, Saito R, Nakajima Y, Ohmiya Y, Yamasaki K, Aiba S (2011) An in vitro test to screen skin sensitizers using a stable THP-1-derived IL-8 reporter cell line, THP-G8. Currently, there is a strong tendency to use biodegradable and safe natural products in formulations that could prevent skin cancer. https://doi.org/10.1093/toxsci/kfr237, The Global Cancer Observatory 2019 Colombia 2018. https://gco.iarc.fr/today/data/factsheets/populations/170-colombia-fact-sheets.pdf, accessed 6 February 2019, Truong VD, Mcfeeters RF, Thompson RT, Dean LL, Shofran B (2007) Phenolic acid content and composition in leaves and roots of common commercial sweetpotato (Ipomoea batatas L.) cultivars in the United States. Am. 16:00h. These phytochemicals displayed anti-inflammatory properties downregulating the NF-κB pathway signaling involved in the IL-8 gene transcription (Liu et al. The main objective of this study is to ascertain whether Ipomoea carnea is a suitable raw material for the production of activated carbon, the quality of the carbon produced and its effectiveness in the removal of copper from aqueous solutions. Ipomoea horsfalliae Hook., Convolvulaceae, called “morning glory,” has simple and dark green alternate leaves; its inflorescences have deep fuchsia color and grow on the terminal part of the branches (Delgado et al. Ipomoea grandifolia and I. nil. are annual herbs with sexual reproduction only (Lorenzi, 2000). PubMed Google Scholar. (Ed.). J Immunol Methods 65:55–63. [ Links ], ENDRESS, P. K. Diversity and evolutionary biology of tropical flowers. In relation to I. grandifolia, the bees Apis mellifera, Ancyloscelis sp., Diadasina riparia, and one fly (Tabanidae) in Botucatu and A. mellifera, Augochloropsis artemisia, one Scoliidae and Thygater analis in Jaboticabal were the most effective pollinators. https://doi.org/10.1111/j.1750-3841.2007.00415.x, WHO–Tropical Diseases 2019. [ Links ], VOGEL, S. Blutenbiologische typen als elements der sippengliederrung. Samples were placed on cassettes for histology and suspended in a p-formaldehyde solution (500 ml, 2% w/v in phosphate buffer, 0.1 M, pH 7.2) for 24 h. They were washed with water (3 h), dehydrated in ethanol solutions (500 ml, 70, 80, 96, or 100% v/v) for 2 h, and included in paraffin. Ipomea horsfalliae flower extract chromatogram (extracted ion chromatogram, EIC), obtained by UHPLC-ESI+-Orbitrap-MS. Cyanidin-3-rutinoside, delphinidin-3-glucoside, and kaempferol-3-glucoside were purchased from PhytoLab (Vestenbergsgreuth, Germany). Uses Containers, Flower … Groups A and C were not irradiated. Many other bees can pollinate these three weeds (Table 4), although less effectively. CC50 values were higher than 200 μg/ml for cells derived from hepatocellular carcinoma (HepG-2, 270 ± 80 μg/ml), monocytic leukemia (THP-1 > 500 μg/ml), normal kidney (HEK-293, 340 ± 110 μg/ml), and normal lung (MRC-5, 250 ± 50 μg/ml). https://doi.org/10.1016/0031-9422(91)83056-Q, Maher VM, McCormick JJ, Grover PL, Sims P (1977) Effect of DNA repair on the cytotoxicity and mutagenicity of polycyclic hydrocarbon derivatives in normal and xeroderma pigmentosum human fibroblasts. Don. The supernatants from cell cultures were collected for the determination of IL-8 by using an ELISA Kit (Invitrogen™). And evolutionary biology of tropical flowers tropical and subtropical regions ( Meira et al ( MC ) is most. Self-Incompatibility in this family can reach very large sizes and cover the crowns of numerous trees colorimetric for! Analysis ( Ludwig & Reynolds, 1988 to a Contract for access to Genetic and. 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